Blood Bank Case Study

------------------------------------------------- Analytical Toxicology Background In analytical toxicology especially to dismantle drugs, several(prenominal) methods can be used. The methods can be categorized cup of tea on their principle of assay which are chemical, spectrometric, chromatographic, electrophoretic and immunochemical assays. each principle of analysis consists of virtually proficiencys that can be used. However, immunochemical assay is normally used in drug covering fire overdue to sensitivity, specificity and low bell than other methods. The half-life of drug excessively used in toxicology analysis to helps in healing(p) observe and accessing drug abuse. Introduction Immunoassays are bioanalytical method in which the quantitation of analyte depends on the reply of antigen (analyte) and antibody. (Ibrahim A. Darwish , 2006). Immunoassay principle is based on competitive cover charge which specific antibody is used to break the presence of analy te or its metabolite in the sample tested. This method consists of some techniques which include: I. Radioimmunoassay (RIA) II. Enzyme immunoassay (EIA) III. Enzyme figure immunoassay technique (EMIT) IV. Enyzme cogitate immunoasorbent assay (ELISA) V. Fluorescence polarization immunoassay (FPIA) VI. Cloned enzyme donor immunoassay (CEDIA) I.

Radioimmunoassay (RIA) This technique is no longer commonly used due to spoil disposal issues where high cost of handling and disposal cost. The analyte in the sample is detected victimization radioisotope. In RIA, sample is change integ rity with specific antibody to the tar ask a! nalyte and tagged analyte with radioactive iodine. Analyte in the sample compete with labeled analyte for antibody. da Gamma rejoinder is used to measure the labeled analyte bind with antibody per slender (CPM). II. Enzyme immunoassay (EIA) EIA is similar to RIA but instead of measuring radiation syndrome this technique measure enzyme activity. It consist of signal detection step which the resolving power is measured optically by fluorescence...If you want to get a full essay, order it on our website: OrderCustomPaper.com

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